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Supplemental Figures from SIRT2-Mediated Deacetylation and Tetramerization of Pyruvate Kinase Directs Glycolysis and Tumor Growth

Year: 2023

Type: supplementary-materials

Abstract: Supplemental Figure S1. PKM2 is acetylated by PCAF and Tip60. Supplemental Figure S2. SIRT2-knockdown stable MCF7, MDA-MB-231, and HeLa cell lines showed decreased PK activity and increased glycolysis rate. Supplemental Figure S3. LC-Ms/Ms analysis to detect potentially reversible acetylation sites of PKM2 peptide. Supplemental Figure S4. Sirt2-/--MMT cell growth was inhibited by SIRT2 and PKM2-deacetylation mimic mutants. Supplemental Figure S5. PKM2 deacetylation mimic mutants (K62R and K305R) increased PKM2 tetramer formation.

DOI: 10.1158/0008-5472.22409460

Authors: Seong-Hoon Park, Ozkan Ozden, Guoxiang Liu, Ha Yong Song, Yueming Zhu, Yufan Yan, Xianghui Zou, Hong-Jun Kang, Haiyan Jiang, Daniel R. Principe, Yong-Il Cha, Meejeon Roh, Athanassios Vassilopoulos, David Gius

Language: English

Topic: Sirtuins and Resveratrol in Medicine

Subfield: Geriatrics and Gerontology

Field: Medicine

Domain: Health Sciences

Open Access status: gold

OpenAlex ID: W4361973381

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