Supplementary Methods from Supramolecular Nanoparticles That Target Phosphoinositide-3-Kinase Overcome Insulin Resistance and Exert Pronounced Antitumor Efficacy
Year: 2023
Type: supplementary-materials
Abstract: All chemical reagents were of analytical grade, used as supplied without further purification unless indicated.All reactions were performed under inert conditions unless otherwise indicated.Dichloromethane (DCM), anhydrous DCM, Methanol, Cholesterol, Dimethylamino Pyridine (DMAP), Succinic Anhydride, Sodium Sulfate, Pyridine, 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), L-α-phosphatidylcholine and Sephadex G-25 were purchased from Sigma-Aldrich.PI-103 was purchased from Selleckchem and PI828 was purchased from Tocris Biosciences.1,2-Distearoyl-sn-Glycero-3-Phosphoethanolamine-N-[Amino(Polythylene Glycol)2000] and the mini handheld Extruder kit (including 0.2 μm Whatman Nucleopore Track-Etch Membrane, Whatman filter supports and 1.0 mL Hamiltonian syringes) were bought from Avanti Polar Lipids Inc. Analytical thin-layer chromatography (TLC) was performed using precoated silica gel Aluminium sheets 60 F 254 bought from EMD Laboratories.Spots on the TLC plates were visualized under UV light, and/or by treatment with alkaline permanganate solution followed by heating.MTS reagent was supplied by Promega.Column chromatography was conducted using silica gel (230-400 mesh) from Qualigens. 1 H spectra were recorded on Bruker DPX 400MHz spectrometer.Chemical shifts are reported in δ (ppm) units using residual 1 H signals from deuterated solvents as references.Spectra were analyzed with Mest-Re-C Lite (Mestrelab
Authors: Ashish A. Kulkarni, Bhaskar Roy, Poornima S. Rao, Gregory A. Wyant, Ayaat Mahmoud, Madhumitha Ramachandran, Poulomi Sengupta, Aaron Goldman, Venkata Ramana Kotamraju, Sudipta Basu, Raghunath A. Mashelkar, Erkki Ruoslahti, Daniela M. Dinulescu, Shiladitya Sengupta
Institution: Brigham and Women's Hospital
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: gold
OpenAlex ID: W4362242056