Supplementary Tables 1 and 2, Figures S1-16 from Metastasis Suppressors Regulate the Tumor Microenvironment by Blocking Recruitment of Prometastatic Tumor-Associated Macrophages
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Tables 1 and 2, Figures S1-16. Supplementary Table 1: Gene lists from DAVID analysis of genes downregulated in RKIP derived TAMs with a Benjamini p-value < 0.5 Supplementary Table 2: Gene lists from DAVID analysis of genes upregulated in RKIP+CCRL5 derived (rescue) TAMs with a Benjamini p-value < 0.5 Figure S1: Immunoblot validation of RKIP expression in 1833 (BM1), MDA-MB-436, and 4T1.2 cells Figure S2: A schematic describing the method used for RNAseq analysis to compare metastatic BM1 with non-metastatic BM1+RKIP Figure S3: Tumor growth in orthotopic xenograft BM1 tumors, significance compared using a 2-way ANOVA. Figure S4: GO categories enriched in BM1+RKIP tumors from RNAseq analysis. Figure S5: Quantile-quantile plots for all stromal cell types tested between metastatic BM1 and non-metastatic BM1+RKIP tumors. Figure S6: Relative TAM infiltration in MDA-MD-436 tumors determined by %pos staining cells for F4/80. Figure S7: Flow cytometric analysis to determine the purity and heterogeneity of BM1 isolated TAMs. Figure S8: CCL5 levels measured by ELISA from BM1 cells in culture. Figure S9: CCL5 expression by qRT-PCR of MDA-MD-436 and 4T1.2 overexpressing RKIP. Figure S10?: RKIP and CCL5 immunoblotts from 1833 (BM1) tumors overexpressing the vectors shown. Figure S11: Tumor growth, tumor weight, and correlation between tumor weight and TAM infiltration in BM1 tumors treated with the CCR5 inhibitor Maraviroc. Figure S12: Invasion assay for BM1 cells overexpressing RKIP, CCL5, or both. Figure S13: Images of Raybiotech L308 arrays. Figure S14: Gene expression data of TAM secreted factors in human patient data sets. Figure S15: Correlation between CCL5 and TAM secreted factors in human patient data sets. Figure S16: Heatmap identifying data sets where breast cancer metastasis free survival is significantly stratified by classifier
Language: English
Topic: Immune cells in cancer
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362284509