Supplementary Figures S1-S9 from Fluorophore-NanoLuc BRET Reporters Enable Sensitive In Vivo Optical Imaging and Flow Cytometry for Monitoring Tumorigenesis
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Figures S1-S9. Figure S1. Functional evaluation of the GpNLuc LumiFluor. Figure S2. In vitro characterization of FACS sorted mouse Em-Myc lymphoma and human non-small cell lung cancer (NSCLC) cells. Figure S3. Temporal development of A549 NSCLC xenograft tumors expressing GpNLuc or GpNLuc and the tumor suppressor LKB1. Figure S4. Detection of spontaneous lymph node metastasis from primary lung tumors. Figure S5. In vitro and in vivo characterization of independently derived E?-Myc lymphoma cells stably expressing GpNLuc. Figure S6. Bioluminescence imaging of E?-Myc lymphoma-GpNLuc allografts. Figure S7. A, in vivo dose-response kinetics of GpNLuc signal strength. Figure S8. Quantification of in vivo bioluminescence imaging and confirmation ex vivo. Figure S9. Flow cytometric analysis of inducibly expressed fluorescent proteins in stable lymphoma cell lines.
Authors: Franz X. Schaub, Md. Shamim Reza, Colin A. Flaveny, Weimin Li, Adele M. Musicant, Sany Hoxha, Min Guo, John L. Cleveland, Antonio L. Amelio
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362311287