Supplemental Figure 2 Brain tumor in adult Drosophila reduces lifespan and causes motor defect. from Drosophila Brat and Human Ortholog TRIM3 Maintain Stem Cell Equilibrium and Suppress Brain Tumorigenesis by Attenuating Notch Nuclear Transport
Year: 2023
Type: supplementary-materials
Abstract: (A) Kaplan-Meyer survival analysis of adult female Drosophila shows that brat-RNAi leads to a reduction in mean life expectancy as compared to controls. (B) Adult flies with brain tumor also have reduced negative geotaxis, a test of motor function. (C,D,E,F) PH3 staining in whole brain shows the adult brat-RNAi brain with actively dividing cells, whereas normal brain does not contain dividing cells. N = 20. P<0.0001. (G, H) Low magnification image of control and brat-RNAi brain. Repo-GFP shows significantly higher number tumor-like cells (marked with Tub-cherry) expressing the glial marker Repo in brat-RNAi brain. (I,J) Higher magnification image of brat-RNAi brain showing cells that do not express Tub-cherry, but that show Miranda expression. bratIR is brat-RNAi. Scale bar: 150 um for (C-F), 100 um for (G,H), 10um for (I,J).
Authors: Subhas Mukherjee, Carol Tucker-Burden, Changming Zhang, Kenneth Moberg, Renee Read, Costas Hadjipanayis, Daniel J. Brat
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362320262