Supplemental Figures 1-14 from Targeting Glycolysis through Inhibition of Lactate Dehydrogenase Impairs Tumor Growth in Preclinical Models of Ewing Sarcoma
Year: 2023
Type: supplementary-materials
Abstract: Combined Supplementary Data file containing: Figure S1. Proliferation assays using NCI-737 and NCI-006 in an expanded panel of EWS cell lines. Figure S2. Effects of additional siRNA sequences against LDHA and LDHB in an expanded panel of EWS cell lines. Figure S3. Effects of additional siRNA sequences against EWS-FLI1 on LDHA and LDHB and additional ChIP-seq data. Figure S4. LDH expression, basal activity and in vitro early time course inhibition in EWS cell lines. Figure S5. Steady state levels of pyruvate and lactate and 13C-tracing experiments from 13C-U-glucose. Figure S6. Effects of NCI-737 and NCI-006 on ECAR measurements. Figure S7. Effects of NCI-737 on OCR measurements. Figure S8. Effects of NCI-737 on ECAR measurements of expanded panel of EWS cell lines. Figure S9. Results of oral dosing of LDH inhibitors in EWS xenograft models. Figure S10. Results of IV pilot studies of LDH inhibitors in EWS xenograft models. Figure S11. Additional growth rate and pharmacokinetic data for EWS xenograft models. Figure S12. Additional growth rate and necrosis data for EWS xenograft models. Figure S13. Depiction of of changes in lactate and pyruvate peaks and lactate/pyruvate ratio by hyperpolarized 13C-MR spectroscopy in EWS xenograft models. Figure S14. Measurements of toxicity in animals treated with NCI-737.
Authors: Choh Yeung, Anna E. Gibson, Sameer H. Issaq, Nobu Oshima, Joshua T. Baumgart, Leah D. Edessa, Ganesha Rai, Daniel J. Urban, Michelle S. Johnson, Gloria A. Benavides, Giuseppe L. Squadrito, Marielle E. Yohe, Haiyan Lei, Sandy Eldridge, John Hamre, Tyrone Dowdy, Victor Ruiz-Rodado, Adrian Lita, Arnulfo Mendoza, Jack F. Shern, Mioara Larion, Lee J. Helman, Gordon M. Stott, Murali C. Krishna, Matthew D. Hall, Victor Darley-Usmar, Leonard M. Neckers, Christine M. Heske
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362363134