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Supplementary Figure 6 from Epigenetic Silencing of MicroRNA-34b/c Plays an Important Role in the Pathogenesis of Malignant Pleural Mesothelioma

Year: 2023

Type: supplementary-materials

Abstract: PDF file - 247K, The effect of the silencing of c-MET protein by siRNA on MPM cell lines. siRNAs targeting c-MET were designed with siDirect online software system (http://www.rnai.co.jp/e_index.html). siRNA sequences targeting two different regions of c-MET are as follows (sense and antisense, respectively): si-c-MET-1 (5'-CAUUGCAUUCGAUAUCAGUGA-3' and 5'-ACUGAUAUCGAAUGCAAUGGA-3') and si-c-Met-2 (5'-CAUGUCAACAUCGCUCUAAUU-3' and 5'-UUAGAGCGAUGUUGACAUGCC-3'). Cells were transfected with 20 nM of siRNA using LipofectamineTM RNAiMAX (Invitrogen) according to the manufacturer's protocol. Control cells were treated with RNAiMAX alone or with scramble. Cell viability after siRNA introduction was determined by a MTS assay.

DOI: 10.1158/1078-0432.22443612

Authors: Takafumi Kubo, Shinichi Toyooka, Kazunori Tsukuda, Masakiyo Sakaguchi, Takuya Fukazawa, Junichi Soh, Hiroaki Asano, Tsuyoshi Ueno, Takayuki Muraoka, Hiromasa Yamamoto, Yasutomo Nasu, Takumi Kishimoto, Harvey I. Pass, Hideki Matsui, Nam-ho Huh, Shinichiro Miyoshi

Language: English

Topic: Occupational and environmental lung diseases

Subfield: Pulmonary and Respiratory Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

OpenAlex ID: W4361902899

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