Supplementary Figure 2 from Pharmacokinetic and Pharmacodynamic Analysis of Circulating Biomarkers of Anti-NRP1, a Novel Antiangiogenesis Agent, in Two Phase I Trials in Patients with Advanced Solid Tumors
Year: 2023
Type: supplementary-materials
Abstract: PDF file, 72K, Binding experiments were carried out by surface plasmon resonance (SPR) measurements on a ProteOn XPR36 (Bio-Rad Laboratories) instrument at 25{degree sign}C. For the binding competition experiment, PlGF (20μg/ml) was immobilized at high surface densities (3000-4000 RU) on an activated ProteOn GLC sensor chip using standard amine coupling procedures as described by the manufacturer. Analytes (Nrp1, Anti-Nrp1B) and 1:1 molar ratio mixture of analytes (Nrp1 + Anti-Nrp1B) were injected in phosphate-buffered saline (PBS), 0.005% v/v Tween-20 (pH7.4) at a flow rate of 100μl/min and sensorgrams for association and disassociation phases were recorded. Analytes were injected for 150 s and allowed to disassociate for 450 s. Data was processed with the ProteOn Manager software (version 2.0, Bio-Rad).
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362394443