Supplementary Figure 1 from Oncogenic BRAF(V600E) Promotes Stromal Cell-Mediated Immunosuppression Via Induction of Interleukin-1 in Melanoma
Year: 2023
Type: supplementary-materials
Abstract: PDF file, 473K, (A) Frequencies of IL-1α and IL-1β staining at different disease stages. Sections of a melanoma progression tissue array stained with antibodies specific for IL-1α or IL-1β and visualized by Vector-red immunostaining. Red color indicates positive staining for (C-E) IL-1α or (G-I) IL-1β in representative primary and metastatic tumors. (J) IL-1α or (K) IL-1β from stage 3 lymph node metastasis of tumors with (red) or without (black) the BRAF V600E mutation. Scores indicates the product of the intensity of signal (0-3) and frequency (0-100) of immunoreactive cells. (L) Presence or absence of IL-1α or IL-1β in melanoma cell lines with known BRAF mutational status. Asterisks, in house, or references (ref.) indicate the source of mutational analysis, (first) and the source of the IL-1 analysis (first and last respectively). Methods of BRAF mutational analysis are indicated by P, pyrosequencing; S, Sanger sequencing; Q, sequenome. Bold text indicates cell lines used in this study.
Authors: Jahan S. Khalili, Shujuan Liu, Tania G. Rodríguez-Cruz, Mayra Whittington, Seth Wardell, Chengwen Liu, Minying Zhang, Zachary A. Cooper, Dennie T. Frederick, Yufeng Li, Min Zhang, Richard W. Joseph, Chantale Bernatchez, Suhendan Ekmekcioglu, Elizabeth Grimm, Laszlo G. Radvanyi, Richard E. Davis, Michael A. Davies, Jennifer A. Wargo, Patrick Hwu, Gregory Lizée
Language: English
Topic: Melanoma and MAPK Pathways
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362396498