Supplementary Figure S2 from A Four-Gene Promoter Methylation Marker Panel Consisting of GREM1, NEURL, LAD1, and NEFH Predicts Survival of Clear Cell Renal Cell Cancer Patients
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Figure S2. Validation of up-regulation of gene expression after demethylation with DAC by qRT-PCR for 22 genes. Relative mRNA expression of 22 genes analyzed by qRT-PCR in ccRCC cell lines SKRC1, SKRC52 and SKRC59 treated with either mock or 5μm 5-aza-2'-deoxycytidine (DAC) for 96 hours. Expression levels were analyzed in triplo unless stated otherwise. Error bars indicate the standard error of the mean. * in duplo; ** single values due to technical failure; *** no expression in SKRC1 and SKRC59.
Authors: Iris J.H. van Vlodrop, Sophie C. Joosten, Tim De Meyer, Kim M. Smits, Leander Van Neste, Veerle Melotte, Marcella M.L.L. Baldewijns, Leo J. Schouten, Piet A. van den Brandt, Jana Jeschke, Joo Mi Yi, Kornel E. Schuebel, Nita Ahuja, James G. Herman, Maureen J. Aarts, Fred T. Bosman, Wim Van Criekinge, Manon van Engeland
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362402983