Supplementary Figure S1-S15 from Fratricide of NK Cells in Daratumumab Therapy for Multiple Myeloma Overcome by Ex Vivo–Expanded Autologous NK Cells
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Figure S1: Dara induces IFN-gamma expression in primary human NK cells; Supplementary Figure S2: MM.1S surface expression of CD38 was assessed by flow cytometry; Supplementary Figure S3: Dara promotes NK-mediated ADCC of MM.1S target cells, which correlates to activation of STAT1 and NF-kB signaling; Supplementary Figure S4: Dara fails to increase apoptosis or IFNG expression in CD16(-) NK-92 cells; Supplementary Figure S5: Reduction in relative proportion of NK cells occurs in both peripheral blood and bone marrow of Dara-treated MM patients; Supplementary Figure S6: Dara increases primary NK cell death; Supplementary Figure S7: Separation of daratumumab into F(ab')2 and Fc fragments; Supplementary Figure S8: CD38^â^'/low primary NK cells show lower apoptotic activity than CD38^+ primary NK cells; Supplementary Figure S9: Compared to their CD38^+ counterparts, the CD38^â^'/low subset of NK cells from peripheral blood of healthy donors are more proliferative and have higher levels of cytotoxicity against MM targets; Supplementary Figure S10: Competition between CD38^+ exp. NK cells and MM cells for Dara binding during co-culture; Supplementary Figure S11: Differential expression of surface receptors within CD38^+ and CD38^â^'/low subsets of NK cells; Supplementary Figure S12: Differential expression of GZMB in CD38^+ and CD38^â^'/low NK subsets found within populations of CD56^bright and CD56^dim NK cells; Supplementary Figure S13: Purity of in vitro-expanded NK cells at day 7; Supplementary Figure S14: NK cells expanded (eNK) from Dara-treated MM patient PBMCs show anti-tumor efficacy against MM in an in vivo mouse model; Supplementary Figure S15: Treatment of MM-bearing mice with or without Dara, expanded NK cells (eNK), or their combination.
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4361936735