Work

Supplementary Figure 6 from Acidity Generated by the Tumor Microenvironment Drives Local Invasion

Year: 2023

Type: supplementary-materials

Abstract: PDF file - 50K, Figure S6: Ex vivo comparison of vessel density in stroma vs tumor tissue. Using the Image Scope v7.1 regions of viable tumor were manually selected. 200�m micrometers were placed around the circumference of the tumor region perpendicular to the tumor edge. A second manually selected region was drawn around the perimeter of the micrometers. This effectively segments the entire tissue section into three regions of interest; the tumor, the edge within 200�m of the tumor and the distant stromal region. The Aperio (Vista, CA, USA) microvessel algorithm v1.0 was used with the following parameters (smoothing 2; dark threshold160; light threshold 210; regioning 6; completion 7) to identify the vascular density which was defined as the vessel area divided by total area (�m2).

DOI: 10.1158/0008-5472.22399056.v1

Authors: Veronica Estrella, Tingan Chen, Mark Lloyd, Jonathan Wojtkowiak, Heather H. Cornnell, Arig Ibrahim-Hashim, Kate Bailey, Yoganand Balagurunathan, Jennifer M. Rothberg, Bonnie F. Sloane, Joseph Johnson, Robert A. Gatenby, Robert J. Gillies

Language: English

Topic: Cancer, Hypoxia, and Metabolism

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

OpenAlex ID: W4362419837

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