Supplemental Movie S3- FRAP assay from PLAC8 Localizes to the Inner Plasma Membrane of Pancreatic Cancer Cells and Regulates Cell Growth and Disease Progression through Critical Cell-Cycle Regulatory Pathways
Year: 2023
Type: supplementary-materials
Abstract: In a Plac8-YFP-transfected S2-007 cell, a region of interest (ROI) covering a Plac8-positive membrane area (white rectangle) was photobleached using high-power laser intensities. Recovery of fluorescence in the ROI was recorded and quantified over time. Fluorescence recovery was complete within 100 s. Scale bar: 10 µm.
Authors: Brajesh P. Kaistha, Holger Lorenz, Harald Schmidt, Bence Sipos, Michael Pawlak, Berthold Gierke, Ramona Kreider, Brigitte Lankat-Buttgereit, Melanie Sauer, Lisa Fiedler, Anja Krattenmacher, Bettina Geisel, Johann M. Kraus, Kristopher K. Frese, Sabine Kelkenberg, Nathalia A. Giese, Hans A. Kestler, Thomas M. Gress, Malte Buchholz
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362298143