Supplementary Materials and Methods and Supplementary Figures 1 through 5 from Multinuclear NMR and MRI Reveal an Early Metabolic Response to mTOR Inhibition in Sarcoma
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Materials and Methods. Figure S1. Major genetic alterations in of PI3K/AKT/mTOR pathway in 265 sarcoma tumors as summarized by TCGA using cBioPortal. Figure S2. Protein expression level detected with Western blot. Lactate Dehydrogenase (LDH), phosphorylated-LDH (p-LDH) and beta actin as a loading control. Figure S3. A) Metabolic profile of total and labeled intracellular B) and extracellular pool size of vehicle vs. rapamycin treated GIST-T1, DDLS, JJ012 and CS1. Last column on both panels shows the fractional enrichment of each metabolite analyzed. Figure S4. Bar plots represent the ratio of A) HP alanine/(HP total carbon) and B) HP bicarbonate/(HP total carbon) in CS1 tumor treated for 24 hours with 6% DMSO (black) and 15mg/kg of rapamycin (red). Figure S5. A) Intracellular concentration of ATP and B) NADPH of DMSO control (black) vs. rapamycin (red) treated GIST-T1, DDLS, JJ012 and CS1.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362421673