Supplemental section from SIRT3-Mediated Dimerization of IDH2 Directs Cancer Cell Metabolism and Tumor Growth
Year: 2023
Type: supplementary-materials
Abstract: Figure S1. Starvation decreased mitochondrial acetylation in MCF7 cells; Figure S2. The image of all harvested tumors from immunodeficient mice injected with MCF-shIDH2-IDH2WT, MCF7-shIDH2-IDH2K413R and MCF7-shIDH2-IDH2K413Q cells were presented; Figure S3. Co-expression of IDH2K413Q with Myc or oncogenic RasG12D increases the invasiveness of NIH3T3 cells in soft agar colony formation assay; Figure S4. Quantification of colony sizes (n=4); Figure S5. Exogenous alpha-ketoglutarate or isocitrate alters clonogenic growth and switches cancer cell metabolism in vitro; Figure S6. Decreased production of ROS by mitoTEMPO decreased clonogenic survival of MCF7-shIDH2-IDH2K413Q cells in vitro; Figure S7. Acetylation of IDH2 negatively correlate with SIRT3 protein levels in breast cancer patient samples
Authors: Xianghui Zou, Yueming Zhu, Seong-Hoon Park, Guoxiang Liu, Joseph O'Brien, Haiyan Jiang, David Gius
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362422336