Supplemental Figure S8 from Therapeutically Active RIG-I Agonist Induces Immunogenic Tumor Cell Killing in Breast Cancers
Year: 2023
Type: supplementary-materials
Abstract: 4T1 cells were transfected with SLR20 or OH-SLR20. At 4 hours after transfection, 4T1 cells were washed 5 times, then cultured in serum-free media for 16 hours. 4T1-cultured supernatant was collected, passed through a 0.2ï�m filter, then added neat to cultures of Raw264.7 cells for 30 minutes. Western analysis of RAW264.7 whole cell lysates was used to measure P-STAT1. Cells were transfected, and after 16 h, total RNA was assessed by RT-qPCR to measure expression of the indicated gene.. Each point represents the average of three experimental replicates, N = 3. Midlines are average {plus minus} S.D. Student's T-test.
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362422575