Figure S1 from Deoxycytidine Release from Pancreatic Stellate Cells Promotes Gemcitabine Resistance
Year: 2023
Type: other
Abstract: Fig. S1. Conditioned media does not affect growth of PDAC cells except at 100% supplementation but does protect human PDAC cell lines regardless of P53 status. (A-E) Arbitrary units of resazurin fluorescence representing growth of PDAC cells after 3 days in culture are shown for cells cultured with the indicated supplement of media conditioned for the indicated number of days. Data show three biological replicates +/- SEM. ***, P {less than or equal to} 0.001, ns, not significant (one-way ANOVA with Bonferroni post-tests). (G) Viability of PDAC organoids after the indicated treatment with 0.05 uM gemcitabine, 20% CM, and/or 20 uM dC. Data show three biological replicates +/- SEM. Mean and SEM are indicated on the plot. One-way ANOVA with Bonferroni post-tests reveals no significant difference between gemcitabine treatment and gemcitabine + CM treatment, or gemcitabine + dC treatment. (F) Viability of the indicated cell lines at the indicated doses of gemcitabine with or without CM supplementation. Data show three biological replicates +/- SEM. *, P {less than or equal to} 0.05, **, P {less than or equal to} 0.01, ns, not significant (two-tailed one sample t-test).
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362363174