Figure S7 from Genomic and Epigenomic Landscaping Defines New Therapeutic Targets for Adenosquamous Carcinoma of the Pancreas
Year: 2023
Type: other
Abstract: Whole genome CNV landscape of PDX models profiled by ATAC-seq. A) ASCPs PAX139, PAX217, and PAX265. Vertical arrows highlight CDK6 amplicons and horizontal arrows highlight CDKN2A homozygous deletions. B) PDACs PAX295, PAX297, and PAX300. The X and Y axes in the CGH plots represent chromosome and log2 ratios for each genome. IGV views of ATAC-seq profiles of C) KRAS; D) MYC and ; E) CDK6 in ASCP (top rows) and PDAC (bottom rows) PDX models. F) Dose response profiles of PAX139 organoids to Palbociclib (left panel) and Abemaciclib (right panel). Organoids were seeded in 96-well plate coated with Matrigel (5,000 cells /well) in organoid media. After 2-3 days of growth, fresh organoid media and DMSO or drug (10 wells/condition) was added. Fresh media and drug was added every 2-3 days. MTT assay were done on day 7 to measure proliferation. The absorbance listed for each drug is the average of the 10 wells per condition. Each experiment was performed in triplicate.
Authors: Elizabeth Lenkiewicz, Smriti Malasi, Tara L. Hogenson, Luis F. Flores, Whitney Barham, William J. Phillips, Alexander S. Roesler, Kendall R. Chambers, Nirakar Rajbhandari, Akimasa Hayashi, Corina E. Antal, Michael Downes, Paul M. Grandgenett, Michael A. Hollingsworth, Derek Cridebring, Yuning Xiong, Jeong-Heon Lee, Zhenqing Ye, Huihuang Yan, Matthew C. Hernandez, Jennifer L. Leiting, Ronald M. Evans, Tamas Ordog, Mark J. Truty, Mitesh J. Borad, Tannishtha Reya, Daniel D. Von Hoff, Martin E. Fernandez-Zapico, Michael T. Barrett
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362423654