Supplementary Figure 1 from Development and Characterization of a Novel CD19CherryLuciferase (CD19CL) Transgenic Mouse for the Preclinical Study of B-Cell Lymphomas
Year: 2023
Type: supplementary-materials
Abstract: PDF file, 432KB, PCR genotyping analysis of CD19cherryluciferase transgenic founders. PCR products of the targeted and wild type alleles of the CD19 locus in two transgenic founders. The PCR conditions for the left arm insertion were: 95{degree sign}C for 5 min; followed by 35 cycles of 95{degree sign}C for 30 sec, 68{degree sign}C for 30 sec, and 68{degree sign}C for 2 min and ending with 68{degree sign}C for 7 min. The PCR conditions for the right arm insertion were: 95{degree sign}C for 5 min; followed by 35 cycles of 95{degree sign}C for 30 sec, 68{degree sign}C for 30 sec, and 68{degree sign}C for 5 min and ending with 68{degree sign}C for 7 min.
Language: English
Topic: CAR-T cell therapy research
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362393582