supplemental figures from Evaluating Acetate Metabolism for Imaging and Targeting in Multiple Myeloma
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Figure 1 A) 1H NMR spectra (CPMG) of conditioned medium from human U266 MMC cells upon incubation for 0 (bottom), 4 (middle) or 18 (top) hours with 10 mM acetate. t-But= t-butanol standard, Lac= lactate (1.31-1.32 ppm), A= alanine (3-position at 1.5 ppm), Acetate=acetate (1.91 ppm), Glu=glutamate (2.34-2.38 ppm), Glc = glucose (5.24 ppm). Supplementary Figure 2 A) 1H NMR analysis in CPMG (top) and gHSQC (bottom) sequences of intracellular extracts from U266 upon 8h incubation with 13C-Acetate 10mM with CHC 2.5 mM (left) or vehicle (DMSO, right). Arrows: acetate peak (1.91 ppm) in HSQC sequences (13C-labeled). Supplementary Figure 3 A) Optical (GFP) and 11C-Acetate PET/CT in KaLwRij mice with bi-lateral flank subcutaneous 5TGM1 tumors (T1 and T2): imaging (i) and TAC (ii) of tumors vs. muscle, p<0.01 by ANOVA. Supplementary Figure 4 A) Percentage of dead cells (7-AAD positive) in tissue homogenates from subcutaneous MM tumors of bortezomib-treated mice relative to vehicle-treated tumors or non-tumor-bearing tissues of both treatment groups (ANOVA, Tuckey's multiple comparison test, ** p<0.01)
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362432797