Supplementary Figures S1-10 from Tumor-Suppressive miR148a Is Silenced by CpG Island Hypermethylation in IDH1-Mutant Gliomas
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Figures S1-10. Figure S1. RRBS methylation results of full miR-148a-associated CpG island. Figure S2. The methylation status of IDH1 mutation is prognostic in GBM and Anaplastic Astrocytoma (Grade III). Figure S3. miR-148a promotor CpG island was demethylated and expression was upregulated after demethylating agent 5-aza-CdR treatment. Figure S4. Knockdown of DNMT1 in U251 cells by using siRNA. Figure S5. Introduction of mutant IDH1 into HEK293T. Figure S6. Overexpression of miR-148a in 293T-IDH1MUT doesn't block the methylation of miR-148a and RBP1. Figure S7. miR-148a expression level in miR-148a stably transfected U251 and T98G cells. Figure S8. Cell cycle analysis of T98G-miR-148a stable cells. Figure S9. The expression of G-CIMP genes DLC1 and CIDEB are downregulated in IDH1MUT GBM patient tissue samples and glioma cell lines. Figure S10. Model summarizing the role of miR-148a in IDH1 mutant gliomas.
Language: English
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362432840