Supplementary Figure 2 from Oncogenic BRAF(V600E) Promotes Stromal Cell-Mediated Immunosuppression Via Induction of Interleukin-1 in Melanoma
Year: 2023
Type: supplementary-materials
Abstract: PDF file, 303K, IL-1α production is reduced by BRAF knockdown shRNA expression in melanoma cells. IL-1α staining in WM793 melanoma cells transduced with scrambled shRNA vector, BRAF targeting shRNA vectors, or untranduced as indicated. Expression of shRNA is indicated by coexpression of RFP (x-axis) and is induced by treatment with 2 ug/mL Doxycycline (DOX) (Lower). Cells were treated with DOX for one week prior to flow analysis by intracellular staining for IL-1α.
Authors: Jahan S. Khalili, Shujuan Liu, Tania G. Rodríguez-Cruz, Mayra Whittington, Seth Wardell, Chengwen Liu, Minying Zhang, Zachary A. Cooper, Dennie T. Frederick, Yufeng Li, Min Zhang, Richard W. Joseph, Chantale Bernatchez, Suhendan Ekmekcioglu, Elizabeth Grimm, Laszlo G. Radvanyi, Richard E. Davis, Michael A. Davies, Jennifer A. Wargo, Patrick Hwu, Gregory Lizée
Language: English
Topic: Melanoma and MAPK Pathways
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362433415