Supplemental Figure 3 from MHC Class I Loss Is a Frequent Mechanism of Immune Escape in Papillary Thyroid Cancer That Is Reversed by Interferon and Selumetinib Treatment In Vitro
Year: 2023
Type: supplementary-materials
Abstract: Supplemental Figure 3. Effect of specific BRAFV600E or radiation on PTC cell line MHC class I expression. (A) The effect of in vitro treatment of PTC cell lines with BRAFV600E-specific tyrosine kinase inhibitors vemurafenib or PLX 4720 on HLA-ABC expression as measured by flow cytometry. (B) The effect of 30 or 60Gy radiation treatment on HLA-ABC expression by PTC cell lines as measured by flow cytometry with data shown as mean +SEM. Representative flow cytometry histograms for HLA-ABC expression are shown to the right. (C) T cell activation measured as the CD25+ fraction of CD3+ T cells or (D) IL-2 production in PBL-PTC cell line co-cultures after PTC tumor cells were pre-treated with radiation. For C and D, data shown are mean (n=4) +SEM, with statistically significant difference from vehicle-treated PTC indicated by *** p<0.001.
Language: English
Subfield: Endocrinology, Diabetes and Metabolism
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362433917