Year: 2023
Type: article
Abstract: Imaging large, cleared samples requires microscope objectives that combine a large field of view (FOV) with a long working distance (WD) and a high numerical aperture (NA). Ideally, such objectives should be compatible with a wide range of immersion media, which is challenging to achieve with conventional lens-based objective designs. Here we introduce the multi-immersion 'Schmidt objective' consisting of a spherical mirror and an aspherical correction plate as a solution to this problem. We demonstrate that a multi-photon variant of the Schmidt objective is compatible with all homogeneous immersion media and achieves an NA of 1.08 at a refractive index of 1.56, 1.1-mm FOV and 11-mm WD. We highlight its versatility by imaging cleared samples in various media ranging from air and water to benzyl alcohol/benzyl benzoate, dibenzyl ether and ethyl cinnamate and by imaging of neuronal activity in larval zebrafish in vivo. In principle, the concept can be extended to any imaging modality, including wide-field, confocal and light-sheet microscopy.
Source: Nature Biotechnology
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $11,690
Funders: National Science Foundation, European Commission, Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, Nederlandse Organisatie voor Wetenschappelijk Onderzoek, Universität Zürich, Horizon 2020 Framework Programme, National Institute of Neurological Disorders and Stroke, H2020 Marie Skłodowska-Curie Actions
Awards: G1137844791, CRISPR/Cas9 based kidney disease modeling to elucidate novel genetic drivers and therapeutic targets in X. tropicalis, G2189472788, CRISPR/Cas9 based kidney disease modeling to elucidate novel genetic drivers and therapeutic targets in X. tropicalis, Inter-areal Neocortical Dynamics during Mouse Behaviour, G434337789, G4937468798, Multi-area two-photon microscopy for revealing long-distance communication between multiple local brain circuits, G5992600816, G6401766094, G6455018820, G6847590645, Short-term memory in distinct reverberating thalamocortical loops, G7358480839, G8271392683, Conserved transcriptional networks of tubulogenesis – towards a mechanistic understanding of renal malformations.
OpenAlex ID: W4362458252