Supplementary Figure 3 from Characterization of the Mechanism of Action of the Pan Class I PI3K Inhibitor NVP-BKM120 across a Broad Range of Concentrations
Year: 2023
Type: supplementary-materials
Abstract: PDF file, 53KB, A and B. 5x105 A2058 cells were seeded in 10 cm dishes and incubated for 24 h either with increasing amounts of BKM120 (A) or with 5 �M of either GDC-0941 (B, top panel) or BEZ235 (B, bottom panel). Cells were then fixed, prepared as described for quantification of the population in the different phases of the cell cycle by fluorescence-activated cell sorting. G1, S and G2/M distribution for control untreated cells are described in the mean text and in Figure 4A. The activities of BKM120 on the cell cycle were plotted along to the inhibitory effects on pAkt levels (A).
Authors: Saskia M. Brachmann, Julia Kleylein-Sohn, Swann Gaulis, Audrey Kauffmann, Marcel J.J. Blommers, Malika Kazic-Legueux, Laurent Laborde, Marc Hattenberger, Fabian Stauffer, Juliane Vaxelaire, Vincent Romanet, Chrystèle Henry, Masato Murakami, Daniel Alexander Guthy, Dario Sterker, Sebastian Bergling, Christopher Wilson, Thomas Brümmendorf, Christine Fritsch, Carlos Garcia-Echeverria, William R. Sellers, Francesco Hofmann, Sauveur-Michel Maira
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362478604