Supplementary Figure 4 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia
Year: 2023
Type: supplementary-materials
Abstract: (A) Kelly cells stably expressing non-targeting shRNA (shNT) or SHMT2 shRNA (shSHMT2) were exposed to normoxia (Nor) or 0.5% O2 (Hyp) for 24 hours. Total cellular DNA were extracted and relative mitochondria/nuclear DNA ratio were measured using Q-PCR. (Data represent Mean {plus minus} S.D of four independent experiments). (B) Kelly cells were exposed to normoxia (Nor) or 0.5% O2 (Hyp) for 6h. ROS levels were measured using a flow cytometry-based MitoTracker Red CM-H2Ros assay. All values are normalized to normoxic shNT control cells (Data represent Mean {plus minus} S.D of three biological repeats, ***p<0.001, determined by Student's two-tailed t-test). (C) Knockdown of SHMT2 display swollen mitochondria upon hypoxic stress. Kelly cells was exposed to normoxia (Nor) or 0.5% O2 (Hyp) for 24 hours. Cells were fixed and pictures were taken under electronic microscope (EM) (25000X). Arrows indicate mitochondria.
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362482444