Supplementary Figures S1-S11 from Cancer-Specific Production of N-Acetylaspartate via NAT8L Overexpression in Non–Small Cell Lung Cancer and Its Potential as a Circulating Biomarker
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Figure S1. Identical exact masses of MMG and MMG-L from HCC4017. Supplementary Figure S2. Confirmation of MMG-L as NAA with LC-MS/MS. Supplementary Figure S3. Confirmation of MMG-L as NAA by comparing its GC MS data (top panel) with that of standard NAA (bottom panel). Supplementary Figure S4. Gene expression levels of NAT8L, the key enzyme for NAA biosynthesis, in 16 squamous cell carcinoma (SC) lung tumors as compared with patient-matched non-malignant lung tissues. Supplementary Figure S5. Gene expression levels of NAT8L in tumors and non-tumor regions of lung cancer patients. Supplementary Figure S6. Western Blot analysis of NAT8L in NSCLC cell lines. Supplementary Figure S7. Dependence of NAA biosynthesis on NAT8L enzyme in HCC4017 lung cancer cells. Supplementary Figure S8. Comparison of intracellular levels of NAA with those of glutamine, glucose, or NAT8L proteins in NSCLC cell lines. Supplementary Figure S9. Comparison of NAAG and NAA levels in HCC4017 cells using LC-MS. Supplementary Figure S10. Standard curve for NAA blood concentrations vs normalized NAA intensities. Supplementary Figure S11. Age-dependent variation of NAA blood levels in control human samples.
Authors: Tzu-Fang Lou, Deepa Sethuraman, Patrick Dospoy, Pallevi Srivastva, Hyun Seok Kim, Joongsoo Kim, Xiaotu Ma, Pei-Hsuan Chen, Kenneth E. Huffman, Robin E. Frink, Jill E. Larsen, Cheryl Lewis, Sang-Won Um, Duk-Hwan Kim, Jung-Mo Ahn, Ralph J. DeBerardinis, Michael A. White, John D. Minna, Hyuntae Yoo
Language: English
Subfield: Biochemistry
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362484690