Supplemental Figure 2: from β-Arrestin-2 Counters CXCR7-Mediated EGFR Transactivation and Proliferation
Year: 2023
Type: supplementary-materials
Abstract: A: Prostate cancer cells were seeded in 6 well plates. Twenty-four hours later, cells were treated with Geldanamycin (3 μM) for 1-24 hr, or were left untreated (NT). Protein extracts were subjected to western blotting. Actin served as a loading control. B: PC-3 cells were seeded in 6 well plates. Twenty-four hours later, cells were treated with 17-AAG (2 μM, 2 hr), proteasome inhibitor MG132 (10 µM, 2 hr) or were left untreated (NT). Protein extracts were collected and subjected to western blotting. Actin served as a loading control. C, D: PC-3 or LNCaP cells were treated with Geldanamycin as described above and nuclear or total protein extracts we collected and subjected to western blot analysis for detection of β-AR2 (C) and EGFR (D). AU: Arbitrary Units
Authors: Georgios Kallifatidis, Daniel Munoz, Rajendra Kumar Singh, Nicole Salazar, James J. Hoy, Bal L. Lokeshwar
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362485681