Figure S3 from Novel Regulation of Integrin Trafficking by Rab11-FIP5 in Aggressive Prostate Cancer
Year: 2023
Type: other
Abstract: Supplementary Figure 3. Recycling kinetics and cell membrane expression of β4 integrin in Rab11-FIP5 depleted cells. DU145 cells were transfected with non-targeting siRNA (siCon), and siRNA against Rab11-FIP5 (siFIP5) or α6 integrin (siα6). (A) Recycling kinetic curve for β4 integrin in untreated and siFIP5 DU145 cells. Percent label recycled to the cell surface at 25{degree sign}C (calculated as percent mean peak fluorescence of recycled label at a given timepoint vs total label internalized) is plotted for different time intervals. First order kinetic curve is fitted (R2>0.98). (B) Maximum label recycled (%) and recycling rate constants (observed, kobs and actual, kactual) calculated as per first order rate kinetics. Results represent 5 independent experiments. Statistical significance calculated for changes in label recycled at each timepoint and maximum label recycled as per student's t-test, unpaired, **p<0.01, n=5. (C) Flow histogram (top) and relative mean peak fluorescence of cell surface expression of β4 integrin assessed by immunolabelling in fixed, non-permeabilized untreated (shaded solid line), siCon (solid line), siFIP5 (dashed line) and siα6 (dotted line) cells (**p<0.01, n=5).
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362486427