Supplemental Material from p53 Promotes Cell Survival due to the Reversibility of Its Cell-Cycle Checkpoints
Year: 2023
Type: supplementary-materials
Abstract: Supplemental Material. Supplemental Figure S1. A: Doxorubicin treatment leads to the concentration-dependent induction of p53 and p21 in parental U2OS cells. U2OS cells were treated with doxorubicin at concentrations ranging from 0.05 μg/ml to 0.2 μg/ml for 24 hours and harvested. Cell extracts were analyzed by immunoblotting for expression of p53, p21, and β-actin (loading control). B: Responses of control and p53-ablated cells to doxorubicin are not clonal in nature. U2OS derivatives expressing either a control shRNA (clone 1 and clone 4) or p53 shRNA (clone 7 and clone 8) were untreated or treated with 0.05 μg/ml doxorubicin for 1-6 days, and then analyzed by flow cytometry. The percentage of cells in each phase of the cell cycle or with a DNA content of less than 2N (
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362486630