Supplementary Figures 1-10 from Multiple Isoforms of CDC25 Oppose ATM Activity to Maintain Cell Proliferation during Vertebrate Development
Year: 2023
Type: supplementary-materials
Abstract: PDF file - 2.3MB, Supplementary Figures and Legends S1. Standstill mutants exhibit persistent growth defects and eventual embryonic death. S2. In situ hybridization of cdc25a in 36 hpf zebrafish embryos. S3. Knockdown of cdc25a by a translation-blocking morpholino phenocopies the standstill phenotype. S4. Zebrafish exhibit a robust cell-cycle arrest following treatment with ionizing radiation, as evaluated by anti-pH3 immunohistochemistry. S5. Inhibition of ATM with the ATM inhibitor KU55933 leads to radiosensitivity in zebrafish. S6. Inhibition of DNA damage response pathways with caffeine leads to increased cell proliferation in cdc25a-/- embryos. S7. The G1/S checkpoint is not activated in cdc25a-deficient embryos. S8. Loss of proliferation precedes the appearance of �H2AX. S9. Overexpression of Cdc25A does not lead to decreased ATM function. S10. Deleterious effects of ATM knockdown.
Language: English
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362487120