Work

Supp. Figure 5 from Targeted AKT Inhibition in Prostate Cancer Cells and Spheroids Reduces Aerobic Glycolysis and Generation of Hyperpolarized [1-13C] Lactate

Year: 2023

Type: other

Abstract: Hyperpolarized MRS of sodium alginate thread cultures. (A) Bright-field image of an alginate thread in culture and (B) H&E stain of the same thread and (C) another cross-section stained with Ki-67. (D) Representative images of an alginate thread cultured in a rotating plate over a period of 4 days and stained with Ki67 All scale bars represent 500 μm. (E) Thread cultures wound on a custom-printed 3D insert, directly deposited into a 5mm NMR tube metabolizes hyperpolarized [1-13C] pyruvate to lactate after injection of 1mM hyperpolarized pyruvate (F). Quantification of integrals of the lactate and pyruvate hydrate peaks over time (G).

DOI: 10.1158/1541-7786.22512915.v1

Authors: Sui Seng Tee, Izabela Suster, Steven Truong, Sangmoo Jeong, Roozbeh Eskandari, Valentina DiGialleonardo, Julio A. Alvarez, Hannah N. Aldeborgh, Kayvan R. Keshari

Language: English

Topic: Cancer, Hypoxia, and Metabolism

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

OpenAlex ID: W4362493569

Cites:

Cited by:

Related to: 10