Work

Supplemental Material, Figures 1, 2 and 3 from Spindle Assembly Disruption and Cancer Cell Apoptosis with a CLTC-Binding Compound

Year: 2023

Type: supplementary-materials

Abstract: S1: Results from AK306 testing on the NCI60 cancer cell line panel. Testing was done by the National Institutes of Health Developmental Therapeutics Program. S2: AK3-BODIPY arrests YAMC cells in mitosis at 600 and 800 nM. YAMCs were treated with compound overnight and then assayed for mitotic arrest by staining for phospho-histone H3. S3: Tumor-bearing ApcÃŽâ€�14/+ mice were treated with five injections of AK306 (30 mg/kg) over the course of three days. Animals were sacrificed and tissue analyzed for PCNA staining (A) and phospho-histone H3 staining (B). Representative staining images are shown in the right panels, with quantified staining shown in the left panels.

DOI: 10.1158/1541-7786.22512910.v1

Authors: Michael J. Bond, Marina Bleiler, Lauren E. Harrison, Eric W. Scocchera, Masako Nakanishi, Narendran G-Dayanan, Santosh Keshipeddy, Daniel W. Rosenberg, Dennis L. Wright, Charles Giardina

Language: English

Topic: Microtubule and mitosis dynamics

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Funders: National Science Council, National Institutes of Health, National Cancer Institute

OpenAlex ID: W4362493607

Cites:

Cited by:

Related to: 10