Supplementary Figures 1-13 from A c-Myc Activation Sensor-Based High-Throughput Drug Screening Identifies an Antineoplastic Effect of Nitazoxanide
Year: 2023
Type: supplementary-materials
Abstract: PDF file - 1.5 MB, Fig. S1. Scheme of the c-Myc activation sensor; Fig. S2. Optimal cell plating numbers for HTS format; Fig. S3. Optimal substrate concentration and imaging time for HTS format; Fig. S4. Robustness of the cell assay; Fig. S5. Plot of the occurrence frequency of compound classes for all the clean hits from five libraries; Fig. S6. The percent inhibition at 10�M of all the clean hits from the NCC library and the IC50 of all the clean hits from the Lopac library are plotted; Fig. S7. Plot of IC50 of all the clean hits from combined MS, BMI and BMF libraries; Fig. S8. The top three unexpected potent hits. The chemical structures of the three most potent hits that were not previously described to have an inhibitory effect on c-Myc are shown; Fig. S9A. Western blot analysis of lymphoma and sarcoma cells after NTZ treatment was performed using phospho c-Myc, c-Myc protein and �-actin antibodies; Fig. S9B. Dose dependent inhibition of phospho c-Myc by NTZ in different cancer cell lines; Fig. S10. Effect of NTZ on cell viability; Fig. S11. In vivo efficacy of NTZ in a SK-FST xenograft model; Fig. S12. Plot of the average body weight of NTZ or CMC treated group at indicated days after implantation.
Language: English
Subfield: Computational Theory and Mathematics
Field: Computer Science
Domain: Physical Sciences
Open Access status: gold
OpenAlex ID: W4362494084