Supplementary Data from Target Deconvolution of a Multikinase Inhibitor with Antimetastatic Properties Identifies TAOK3 as a Key Contributor to a Cancer Stem Cell–Like Phenotype
Year: 2023
Type: supplementary-materials
Abstract: Suppl. Fig. 1: Procedures for synthesis of NCGC00188382 (Inhibitor#1) Suppl. Fig. 2: qPCR array of 96 stem cell genes from PANC1 adherent and PANC1 spheroids reveal up-regulation of six stem cell genes in organotypic Panc1 spheres. Suppl. Fig. 3: Inhibitor #1 induces apoptosis in KLM-1 spheroids. Flow cytometry measuring fraction of cells with exposed extracellular phosphatidylserine via annexin V-FITC (in PI stained cells; y-axis). Suppl. Fig. 4: In vivo activity of inhibitor #1. Suppl. Fig. 5: Inhibitor #1 suppresses downstream signaling of aurora kinase B, and ephrin B2 receptor activation identified as targets within in situ kinome screen. Suppl. Fig. 6: Outcomes of combination drug screening of inhibitor #1 versus a collection of approved and investigational drugs. Suppl. Fig. 7: Confirmation of synergies derived from 10Ã-10 matrix screening with inhibitor #1 in PANC1 spheroids. Suppl. Fig. 8: A. Flow cytometry measuring DNA breaks and fragments after labeling of 3'-hydroxyl ends with bromolated deoxyuridine triphosphate nucleotides (Br-UTP) after 96-hour treatment with #1 in PANC1 spheroids and 2D monolayer cells.
Language: English
Topic: Cancer Cells and Metastasis
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362482201