Data from Clinical Utility of a Blood-Based BRAFV600E Mutation Assay in Melanoma
Year: 2023
Type: other
Abstract: BRAF inhibitors (BRAFi) have led to clinical benefit in patients with melanoma. The development of a blood-based assay to detect and quantify BRAF levels in these patients has diagnostic, prognostic, and predictive capabilities that could guide treatment decisions. Blood BRAFV600E detection and quantification were performed on samples from 128 patients with stage II (19), III (67), and IV (42) melanoma. Tissue BRAF analysis was performed in all patients with stage IV disease and in selected patients with stage II and III disease. Clinical outcomes were correlated to initial BRAF levels as well as BRAF level dynamics. Serial analysis was performed on 17 stage IV melanoma patients treated with BRAFi and compared with tumor measurements by RECIST. The assay was highly sensitive (96%) and specific (95%) in the stage IV setting, using a blood level of 4.8 pg as “positive.” BRAF levels typically decreased following BRAFi. A subset of these patients (5) had an increase in BRAFV600E values 42 to 112 days before clinical or radiographic disease progression (PD). From 86 patients with resected, stage II or III melanoma, 39 had evidence of disease relapse (45.3%). Furthermore, BRAF mutation in the blood after surgical resection in these patients was not associated with a difference in relapse risk, although tissue BRAF status was only available for a subset of patients. In summary, we have developed a highly sensitive and specific, blood-based assay to detect BRAFV600 mutation in patients with melanoma. Mol Cancer Ther; 13(12); 3210–8. ©2014 AACR.
Authors: David J. Panka, Elizabeth Buchbinder, Anita Giobbie-Hurder, Aislyn P. Schalck, Laleh Montaser-Kouhsari, Alireza Sepehr, Donald P. Lawrence, David F. McDermott, Rachel Cohen, Alexander Carlson, Jennifer A. Wargo, Ryan Merritt, Virginia J. Seery, F. Stephen Hodi, Anasuya Gunturi, Dennie Fredrick, Michael B. Atkins, A. John Iafrate, Keith T. Flaherty, James W. Mier, Ryan J. Sullivan
Language: English
Topic: Melanoma and MAPK Pathways
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362495452