Supplementary Video 2 from The Acetylenic Tricyclic Bis(cyano enone), TBE-31 Inhibits Non–Small Cell Lung Cancer Cell Migration through Direct Binding with Actin
Year: 2023
Type: supplementary-materials
Abstract: PDF - 8699K, Single cell tracking of NIH 3T3 cells was carried out using time-lapse microscopy with an Olympus IX81 inverted microscope equipped with a custom chamber which allowed us to maintain a temperature of 37 degrees C and an atmosphere containing 5% CO2. Bright field images were collected from cells incubated with vehicle (DMSO), 1 microM TBE-31 or 0.5 microM CDDO-Im at 10 min intervals over 18 h. Distance of migration was determined by tracking the positions of cell nuclei over 18 h using the MtrackJ plugin (32) for imageJ software (33).
Authors: Eddie Chan, Akira Saito, Tadashi Honda, Gianni M. Di Guglielmo
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362511820