Supplementary Figures S1-S5 from A New Class of Antibody–Drug Conjugates with Potent DNA Alkylating Activity
Year: 2023
Type: supplementary-materials
Abstract: Figure S1. Comparison of the rate of adduct formation of the indolinobenzodiazepine dimer 6 and the pyrrolobenzodiazepine dimer SJG-136 with dsDNA prepared from annealing of single strand DNA 3'-TATAGATCTATA-5'. Figure S2. A, In vitro potency of anti-EpCAM ADC 1a toward antigen (+) HCT-15 cells without or with addition of blocking antibody. Figure S3. A, Structures of IGNs used in the DNA adduct formation assay. B, Time-dependent reversal of IGN-DNA adducts isolated from EOL-1 cells that had been exposed to the IGN diimine 7 or the monoimine 8, after heating to 90{degree sign} C. C, The ability of IGN 7 (diimine) and 8 (monoimine) to crosslink DNA was tested in an acellular comet assay with nuclear DNA from human normal lymphocytes. Figure S4. In vitro potency of anti-FRα ADC 4a toward antigen (+) KB cells without or with addition of an excess (1 x 10-6 M) blocking antibody. Figure S5. Comparison of the in vitro potency of IGN 9 and its sulfonated version towards the human Burkitt cell line Namalwa.
Language: English
Subfield: Organic Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
OpenAlex ID: W4362527627