Supplementary Methods, Supplementary Figures 1-3, Supplementary Tables 1-9 from DOT1 L Regulates Ovarian Cancer Stem Cells by Activating β-catenin Signaling
Year: 2023
Type: supplementary-materials
Abstract: S1. DOT1L is important for stemness in OC cells. S2. Inhibition of DOT1L activity reduces ALDH+ ovarian CSCs. S3. Inhibition of DOT1L activity inhibits Wnt pathway. Table S1. Cell culture conditions Table S2. Antibodies Table S3. shRNA sequences Table S4. qRT-PCR Primers Table S5. ChIP-PCR Primers Table S6. Effects of DOT1L knockdown on tumor initiation capacity Table S7. Estimated 1/stem cell frequency in shCtrl and shDOT1L OVCAR5 cells in serial dilution assay experiment. Table S8. Top 35 downregulated genes from the RNA-sequencing analysis of OVCAR5 cells transduced with shRNA targeting DOT1L versus control shRNA. Table S9. Top 35 upregulated genes from the RNA-sequencing analysis of OVCAR5 cells transduced with shRNA targeting DOT1L versus control shRNA.
Language: English
Topic: Cancer Cells and Metastasis
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362530730