Supplementary Figure S4 from Targeting CD20+ Aggressive B-cell Non–Hodgkin Lymphoma by Anti-CD20 CAR mRNA-Modified Expanded Natural Killer Cells In Vitro and in NSG Mice
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Fig.S4: In vitro bioluminescence to detect luciferase expression in Raji and Raji-2R cells Raji and Raji-2R cells were electroporated with ffLUCZeo-pcDNA and selected for stable clones expressing luciferase (Luc) with Zeocin. (A) The bioluminescence intensity increased proportionally with increasing Raji-Luc cell numbers. A strong correlation between number of cells and light emission was obtained (R2= 0.99). (B) The bioluminescence intensity increased proportionally with increasing Raji-2R-Luc cell numbers. A strong correlation between number of cells and light emission was obtained (R2= 0.989). (C)-(D) Dynamic change of the bioluminescent signal in Raji-Luc and Raji-2R-Luc after over 2 months selection. Signal intensities are shown as a function of time after addition of D-luciferin.
Language: English
Topic: CAR-T cell therapy research
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362532377