Work

Figure S5 from Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling

Year: 2023

Type: other

Abstract: Selumetinib fails to recruit CNKSR1 and p-AKT to the plasma membrane in CNKSR1 low cell lines; Proximal ligation of CNKSR1 and p-AKT in CNKSR1 low cell lines treated with vehicle vs selumetinib;CNKSR1 p-AKT PLA signal after treatment with selumetinib is dependent on the presence of both primary antibodies; Silencing of CNKSR1 in the CNKSR1high cell lines abrogates PLA signal; and Immunofluorescence staining of PIP3 and CNKSR1 in YAPC treated with vehicle and selumetinib.

DOI: 10.1158/1541-7786.22552180

Authors: Dandan Li, Anne M. Miermont, Rushikesh Sable, Humair S. Quadri, Lesley A. Mathews Griner, Scott E. Martin, Taivan Odzorig, Soumita De, Marc Ferrer, Astin S. Powers, Stephen M. Hewitt, Udo Rudloff

Language: English

Topic: Protein Kinase Regulation and GTPase Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

OpenAlex ID: W4362533570

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