Supplementary Data from Scavenger Receptor Type B1 and Lipoprotein Nanoparticle Inhibit Myeloid-Derived Suppressor Cells
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Figures S1-15 and Supplementary Tables S1-S2. Supplementary Figure S1: Flow cytometry gating scheme for SCARB1 expression. S2: Flow cytometry gating scheme for HDL NP targeting of CD11b+ cells. S3: HDL NP cytotoxicity toward CD11b+ cells. S4: Flow cytometry gating scheme for T cells and MDSCs. S5: HDL NP effect on MDSC distribution in lymph nodes. S6: HDL NPs inhibit MDSC-mediated suppression of T cell proliferation. S7: HDL NP toxicity toward MDSCs. S8: HDL NP toxicity toward B16F10 melanoma cells. S9: Survival results from mice with orthotopic implantation of B16F10 melanoma. S10: H&E staining of lungs with B16F10 metastases obtained from SCARB1 -/- mice. S11: SCARB1 expression in CD11b+ bone marrow cells after bone marrow transplant with SCARB1 -/- cells. S12: H&E staining of lungs from mice with established metastases at the time of treatment initiation. S13: F4/80 and CD3 staining of lungs from mice with established metastases at the time of treatment initiation. S14: HDL NP toxicity toward LLC cells. S15: Summary of HDL NP mechanisms of reducing MDSC function. Supplementary Table S1: Table of antibodies used in this study. S2: Table of PCR primers used in this study.
Language: English
Topic: Immune cells in cancer
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362534823