Supplementary Table S1 and Supplementary Figures S1-S14 from Tumor-targeted Nanoparticle Delivery of HuR siRNA Inhibits Lung Tumor Growth In Vitro and In Vivo By Disrupting the Oncogenic Activity of the RNA-binding Protein HuR
Year: 2023
Type: supplementary-materials
Abstract: Table S1. Table showing oligonucleotide primer sequence used in RT-PCR Figure S1. Physicochemical characterization of the nanoparticles shows shape, siRNA protection and siRNA release kinetics. Figure S2. Transferrin (Tf) ligand optimization on nanoparticles measured by fluorescence microscopy and fluorescence intensity. Figure S3.Nanoparticle specificity and receptor-mediated uptake studies. Figure S4. Western blotting showing HuRsiRNA4 but not other siRNAs used as controls reduced HuR protein expression. Figure S5. HuR mRNA expression analysis in nanoparticle-treated tumor and normal cells. Figure S6.Semi-quantitative analysis of HuR and HuR-regulated protein expression in A549 cells. Figure S7.Semi-quantitative analysis of HuR and HuR-regulated protein expression in HCC827 cells. Figure S8.Semi-quantitative analysis of HuR and HuR-regulated protein expression in MRC-9 cells. Figure S9. Western blot analysis shows HuR-TfNP treatments induces apoptosis in A549 cells. Figure S10. Molecular imaging showing biodistribution and kinetics of free ICG and ICG-TfNP in tumor-bearing and non-tumor-bearing naive mice respectively. Figure S11. Bioluminescence imaging showing tumor growth measurement over time in A549 tumor-bearing mice. Figure S12. Molecular analysis of HuR-TfNP-treated tumor tissues shows reduction in HuR mRNA and HuR-regulated proteins and induction of apoptosis. Figure S13. Immunohistochemical analysis of HuR-TfNP-treated A549 tumor tissues for changes in morphology, and in expression of HuR, CD31, KI67 and TUNEL positive cells. Figure S14.Immunohistochemical analysis of HuR-TfNP-treated HCC827 tumor tissues for changes in morphology, and in expression of HuR, CD31, and KI67.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362535332