Supplementary Figure 3 from Augmented HR Repair Mediates Acquired Temozolomide Resistance in Glioblastoma
Year: 2023
Type: supplementary-materials
Abstract: Supplemental Figure 3. A-B. U251 cells and their TMZ-treated counterparts (U251-T12) were A) pulsed with 10 μM TMZ for 48 hours, or B) treated with 1 Gy of IR, fixed at the indicated time points post-treatment, immunofluorescence-stained for 53BP1 foci, and imaged at 40X magnification. Representative images are shown. Rates of repair of TMZ-induced DSBs were determined by scoring 53BP1 foci. Average number of 53BP1 foci per nucleus (y-axis) is plotted against the corresponding time post-treatment (x-axis). Error bars, S.E.M. ****, p< 0.0001. C. Sensitivities of U251 and U251-T12 cells to IR were assessed by colony formation assays. The fraction of surviving colonies (y-axis) is plotted against the corresponding TMZ dose (x-axis). Error bars, S.D.
Language: English
Subfield: Genetics (clinical)
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362529170