Supplementary Figure S1 from Mutant Calreticulin Requires Both Its Mutant C-terminus and the Thrombopoietin Receptor for Oncogenic Transformation
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Figure S1. (A) Relative frequency of GFP positive cells in peripheral blood over time in BMT mice receiving empty vector, CALRWT or CALRMUT-expressing c-Kit+ BM cells. (B) Histopathologic H&E sections of BM from representative EV, CALRWT or CALRMUT recipient mice demonstrates megakaryocytic hyperplasia in CALRMUT recipient animals (100X magnification) (C) A N-terminal anti-CALR antibody demonstrated strong, diffuse cytoplasmic reactivity in megakaryocytes in representative normal and CALR-mutant patient bone marrow biopsies. In addition, cytoplasmic reactivity was noted in smaller mononuclear cells, which were morphologically most suggestive of early myeloid forms. Strong reactivity was not observed in mature erythrocytes or in mature granulocytes. (D) Flow cytometric analysis of Lin−Sca-1+c-Kit+ (LSK) and Lin−Sca-1−c-Kit+ (LK) cell numbers in the bone marrow of BMT mice receiving empty vector, CALRWT or CALRMUTexpressing BM cells 16 weeks post bone marrow transplantation demonstrates a significant increase in LSK cells in CALRMUT recipients. All p values were determined by unpaired two-tailed Student's t test. (*0.01 < p < 0.05; (**0.001 < p < 0.01; ns, not significant).
Language: English
Subfield: Genetics (clinical)
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4362538548