Supplementary Figure Legends, Figures 1 - 5 from TGFβ1-Mediated SMAD3 Enhances PD-1 Expression on Antigen-Specific T Cells in Cancer
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Figure 1. The effect of various cytokines on PD-1 expression is shown as fold changes in MFI relative to the αCD3/αCD28 condition with no cytokines in both CD4+ (black bars) 4 and CD8+ (white bars) T cells. Supplementary Figure 2. qPCR analysis of Smad2 and Smad3 mRNA levels in Jurkat T cells. Supplementary Figure 3. Foxp3 expression in CD4+ PD-1+ T cells infiltrating the tumor 56 microenvironment in WT, Smad2 cKO and Smad3 cKO mice. Supplementary Figure 4. Representative histograms of CFSE (left), PD-1(middle) and LAG3 (right) 66 expression on WT (thin grey histogram) and Smad3 (thin black histogram) cKO OT-I cells originating 67 from the draining lymph nodes (DLN) (top) and non-draining lymph nodes (NDLN) (bottom). Supplementary Figure 5. (a) WT and DNTGFβRII Tg+ mice were injected with 105 B16 melanoma 74 cells in foot-pads, and tumor volume (mm3) is shown as mean +/- SEM on different days.
Language: English
Topic: TGF-β signaling in diseases
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362531352