Supplementary Fig. S7 from Salmonella-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors
Year: 2023
Type: supplementary-materials
Abstract: PMN tracking in control mice. (A) Normal C57BL/6 non-tumor bearing mice were injected intravenously with 50 uL of a 20 uM working solution of NS-NIR reagent. Mice were imaged 1 hr proceeding injection of NS-NIR. Mice were also injected with 300 ug D-luciferin in 200 µL PBS 5 min prior to imaging. Image depicts representative mouse. Imaged with a Xenogen IVIS 100. (B) Mice treated with shScr-ST or shIDO-ST alone show no intratumoral PMN infiltration. C57BL/6 mice implanted with orthotopic KPC-luc tumors were treated with shIDO-ST or shScr-ST as outlined in Supplementary Fig. S2. Mice were imaged at the indicated time points following first treatment with ST. Imaged with a Xenogen IVIS 100. (C) Key replicate data of PMN infiltration (brown) and tumor (rainbow) for indicated treatment groups. Imaged with a Spectral Ami-X.
Language: English
Subfield: Biotechnology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362539732