Supplementary Fig. S5 from Salmonella-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors
Year: 2023
Type: supplementary-materials
Abstract: Colony forming unit (CFU) enumeration of ST in tumors of mice treated with and without PEGPH20. Mice with 14-day orthotopic KPC tumors (n=3) were given indicated treatment regimens. Tumors were then extracted at indicated time points post-treatment, homogenized in PBS containing a protease inhibitor mix (Roche), plated on LB-0 plates containing appropriate antibiotics, and then incubated at 37C overnight. Data is representative of multiple experiments utilizing identical treatments and post-treatment analyses. (A) CFU comparison of mice treated with ST, with and without PEGPH20, at various time points after treatment. (B) Effects of Gr-1 depletion (Gr1-) on accumulation of shIDO-ST in orthotopic KPC tumors in mice treated with and without PEGPH20. Mice were depleted 24hr prior to treatment. Tumors were extracted 48hr post-treatment and homogenized for CFU enumeration. *p<0.05, **p<0.01, 1-way ANOVA.
Language: English
Subfield: Biotechnology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362539760