Supplementary Figures S1 to S13 from Combined Pan-RAF and MEK Inhibition Overcomes Multiple Resistance Mechanisms to Selective RAF Inhibitors
Year: 2023
Type: supplementary-materials
Abstract: Figure S1: Following BRAF inhibition, recovery of ERK phosphorylation is observed and RKO cells continue to proliferate in the presence of PLX4720. Figure S2: DAPPLE analysis of the top 300 candidate genes from the primary screen identifies potential protein interaction networks. Figure S3: Knockdown of candidate genes sensitises cells to BRAF inhibition. Figure S4: Inhibition of MET is synergistic with PLX4720. Figure S5: Inhibition of SHP2 sensitises to PLX4720. Figure S6: Combinations of PLX4720 and AZD6244 are not synergistic. Figure S7: Cells displaying acquired resistance to PLX4720 are sensitive to combined pan-RAF and MEK inhibition. Figure S8: The combination of AZD6244 and RAF265 is synergistic. Figure S9: The combination of AZD6244 and MLN2480 is synergistic. Figure S10: The combination of GSK1120212 and AZ628 is synergistic. Figure S11: AZ628 shows greater synergy when combined with a MEK inhibitor than GSK2118436. Figure S12: Synergy between pan-RAF and MEK inhibition is rescued by a resistant allele of MEK. Figure S13: Pan-RAF and MEK inhibition results in decreased cell viability.
Language: English
Topic: Melanoma and MAPK Pathways
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362547634