Supplementary Figure S1 from Dual Inhibition of NOX2 and Receptor Tyrosine Kinase by BJ-1301 Enhances Anticancer Therapy Efficacy via Suppression of Autocrine-Stimulatory Factors in Lung Cancer
Year: 2023
Type: supplementary-materials
Abstract: Effects of BJ-1301 and α-TOH on cell viability, proliferation, and VEGF-induced tube formation. A, Effects of α-TOH or BJ-1301 on the viability of HUVECs and BAOECs were determined using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. B, Effect of BJ-1301 alone treatment on HUVEC proliferation. C, HUVECs plated in a well coated with Matrigel basement membrane matrix, were treated with compounds in the presence of VEGF (20 ng/ml). The total tube length per field was measured using Image Inside software (Upper panel). Invasion of HUVECs was measured using VEGF-induced HUVEC migration in the Transwell assay (Lower panel). The bar graphs represent the mean {plus minus} S.E.M. of three independent experiments. *P<0.05 versus vehicle-treated control. #P<0.05 versus VEGF alone. D, Inhibitory effect of BJ-1301 on VEGF-induced tube formation in a time-dependent manner.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4362548352